The benefits of FSC-A certification include:
Here, "FSC-A" is calculated from the image mask. While less common, the same principle applies: area vs. height (or aspect ratio) weeds out doublets and clusters. However, imaging provides the ultimate confirmation – you can literally see if it’s a doublet.
: If two cells are stuck together (a "doublet"), the machine might count them as one giant cell with double the DNA or protein markers, leading to false data.
Plot FSC-A (X-axis) vs. FSC-H (Y-axis). Draw a polygon tightly around the diagonal population. Alternatively, use FSC-W vs. FSC-A. The singlet gate should exclude events with high FSC-W or mismatched A/H ratios.
: By plotting FSC-A (Area) against FSC-H (Height) , scientists can find single cells.
FSC: A repository project for an international non-profit - DSpace